Proven performance from the industry standard

The GeneChip® HT HG-U133+ PM Array Plate enables high-throughput expression profiling of 24 or 96 samples at a time using the same content as the industry-standard GeneChip® Human Genome U133 Plus 2.0 Array*.

The HT HG-U133+ PM Array Plate enables you to:

• Measure gene expression of more than 47,000 transcripts and variants, including more than 33,000 well-characterized genes and UniGene clusters per sample
• Get accurate and reproducible data by using multiple independent measurements for each transcript

Scatter plots represent median MAQC B - MAQC A signals for each array type. The high correlation coefficients indicate similar biology is being observed.

Key Benefits

• Enables increased productivity and efficiency through parallel processing
  • Process 24 or 96 samples on a single array plate
• Same industry-leading performance and content as the Human Genome U133 Plus 2.0 Array in cartridge format
  • Strong signal and fold change correlation to previous plate and cartridge designs
• Multiple independent measurements per transcript for increased confidence in your results
  • Interrogates more sequence than a single probe measurement
• Conveniently packaged
  • Individual 24- and 96-array plates available
  • All processing trays included

Content Profile

Sequences used in the design of the arrays were selected from GenBank®, dbEST and RefSeq. The majority of sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

An additional set of sequence clusters were created from Build 159 of UniGene (January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31). Sequences were further analyzed for correct orientation, false priming, false clustering, alternative splicing and alternative polyadenylation.

* Two design changes were introduced with the GeneChip HT HG-U133+ PM Array Plate:

• Only perfect match (PM) probes from the cartridge design were retained while mismatch (MM) probes were removed.
• Empirical data was used to select the best-performing probes resulting in reducing the number of PM probes
  • 42,461 probe sets were reduced from 11 to nine probes
  • Six probe sets were reduced from 11 to 10
  
  

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Data Sheets

HT Array Replacement Guideline (pdf, 179 KB)

HT PM Array Plate System for Human, Mouse, and Rat (pdf, 476 KB)

Technical Notes

Performance and Validation of GeneChip® HT Human, Mouse and Rat PM Array Plates (pdf, 678 KB)

White Papers

HT and Cartridge IVT Expression Arrays (pdf, 601 KB)

Part #	Description	Details
901261	GeneChip® HT HG-U133+ PM 24-Array Plate	Contains 1 24-array plate and necessary hybridization, wash and scan trays.
901262	GeneChip® HT HG-U133+ PM 96-Array Plate	Contains 1 96-array plate and necessary hybridization, wash and scan trays.

FAQs

HT PM Plate Arrays

Manuals

Expression Analysis Technical Manual For HT Array Plates Using the GeneChip® Array Station (pdf, 7.4 MB)

Quick Reference Cards

QC Metrics for Human, Mouse and Rat HT PM Array Plates (pdf, 111 KB)

Sample Data

Human Tissue Panel (zip, 216 MB)

Library Files

HT HG-U133+ PM Array Plate (zip, 16 MB)

Package Inserts

HT HG-U133+ PM Array Plate (pdf, 703 KB)

Alignment, Annotation, and Sequence Files

Information about using Alignment, Annotation, and Sequence files

Current NetAffx Annotation Files

HT_HG-U133_Plus_PM Annotations, CSV format (20 MB, 12/1/08)

HT_HG-U133_Plus_PM BLASTP annotations, CSV Format (77 KB, 11/24/08)

Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.

Sequence Files

HT_HG-U133_Plus_PM Probe Sequences, FASTA format (9.6 MB, 7/16/08)

HT_HG-U133_Plus_PM Probe Sequences, tabular format (8.5 MB, 7/16/08)

Archived NetAffx Annotation Files

HT_HG-U133_Plus_PM Annotations, CSV format (20 MB, 9/15/08)

Additional Support

Fluidics Scripts

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